Congratulations to Yuda Huo, for The deubiquitinating enzyme USP46 regulates AMPA receptor ubiquitination and trafficking
Journal of Neurochemistry (2015) J Neurochem 134(6), 1067–1080.
Yuda Huo, Natasha Khatri, Qingming Hou, James Gilbert, Guan Wang and Heng-Ye Man. DOI: 10.1111/jnc.13194
The article has broad relevance in the field of protein ubiquitination, a highly dynamic and reversible process, achieved via the balance between ubiquitination and deubiquitination. The glutamatergic AMPARs, which mediate most of the excitatory synaptic transmission in the brain, are known to be subjected to Nedd4-mediated ubiquitination; however, the deubiquitination process and the responsible deubiquitinating enzymes (DUBs) for mammalian AMPARs remain elusive. We find that AMPARs are subject to K63-type ubiquitination, and identify USP46 as the DUB for AMPARs. USP46 deubiquitinates AMPARs in vitro and in vivo. Up- or down-regulation of USP46 leads to changes in AMPAR ubiquitination, surface expression, and trafficking, as well as the strength of synaptic transmission. USP46-mediated regulation of AMPAR ubiquitination and turnover may play an important role in synaptic plasticity and brain function.
Yuda Huo has co-authored a number of relevant articles in the field in the past, using a variety of biochemical, molecular and cell biological as well as imaging methods. He is currently working at Boston University, USA under the guidance of Dr. Heng-ye Man to pursue his PhD degree in Neuroscience.